MALDI

The Bruker autoflex maX is a MALDI-TOF/TOF mass spectrometer built with more than 25 years of proven MALDI technology, combining a small footprint with the patented smartbeam-II solid-state laser for a wide range of applications. With a laser repetition rate of up to 2 kHz in MS mode and 200 Hz in MS/MS mode, time-sensitive analyses such as biopharmaceutical integrity screening can be completed in minutes. The high ionization efficiency of the smartbeam-II laser minimizes sample consumption while maximizing data quality at each target position, even for complex samples, and its gentle ionization allows tissue samples to be stained after MALDI MS imaging analysis.

Key features:

• Patented smartbeam-II solid-state laser with small instrument footprint

• Laser repetition rate up to 2 kHz in MS mode, 200 Hz in MS/MS mode

• High ionization efficiency minimizes sample consumption

• Gentle ionization allows post-analysis tissue staining for MALDI imaging

• More than 25 years of proven MALDI-TOF technology

Typical applications:

• Rapid discovery and characterization workflows

• LC-free quality control and target characterization

• Biopharmaceutical integrity screening and MALDI MS imaging

The Bruker microflex LRF is the most powerful benchtop MALDI-TOF/TOF system available today, delivering up to 15,000 resolution while remaining easy to use for many analytical challenges. Proprietary PAN pulsed ion extraction, an independent reflector power supply, an integrated MCP detector and high-precision digital converters combine to deliver the best mass resolution of a benchtop system in both positive and negative ionization modes. A gridless reflector together with proprietary AnchorChip technology delivers unmatched sensitivity across analyte types such as oligonucleotides, proteins and polymers, while a robust, oil-free vacuum system and stable ion generation guarantee maximum uptime even in busy, multi-user laboratories.

Key features:

• Up to 15,000 resolution in a benchtop MALDI-TOF/TOF system

• Proprietary PAN pulsed ion extraction and independent reflector power supply

• Integrated MCP detector with high-precision digital converters

• Gridless reflector with AnchorChip technology for unmatched sensitivity

• Oil-free vacuum system for maximum uptime in multi-user labs

Typical applications:

• Analysis of oligonucleotides, proteins and polymers

• Routine and research MALDI-TOF/TOF workflows

• Multi-user, high-throughput laboratory environments

The Bruker rapifleX is the most advanced MALDI-TOF/TOF system on the market, delivering analytical results within minutes thanks to a 10 kHz scanning smartbeam 3D laser that runs up to 20 times faster than traditional MALDI-TOF systems. Adaptable ion optics and class-leading laser technology provide the best data quality regardless of acquisition mode or mass range, while an intuitive interface gets new users up and running quickly. The robust, easy-to-access ion source is designed for straightforward cleaning and maximum uptime in 24/7 operation, and the unprecedented 10 kHz speed, wide dynamic range, and high specificity and resolution support detailed characterization for both tissue imaging and high-throughput primary screening.

Key features:

• 10 kHz scanning smartbeam 3D laser — up to 20× faster than traditional MALDI-TOF

• Adaptable ion optics for the best data quality across all acquisition modes

• Robust, easy-to-access ion source designed for 24/7 operation

• Wide dynamic range with high specificity and resolution

• Intuitive operation for fast start-up and ease of use

Typical applications:

• MALDI tissue and MS imaging

• High-throughput primary screening

• Protein characterization requiring speed and robustness

The Bruker smartfleX brings the speed and versatility of MALDI-TOF to a true benchtop format, using minimum lab space while operating silently and providing reliable answers instantly. It offers access to 100% of the MALDI analyte space through seamless switching between positive and negative ionization modes without any limitations, covering compounds from small molecules up to intact proteins. Bruker's smartbeam laser technology delivers enhanced sensitivity across this wide range of analytes, while an IR-laser-based automated source-cleaning system minimizes instrument downtime, making smartfleX one of the easiest-to-use and most robust MALDI-TOF systems on the market.

Key features:

• True benchtop format using minimum laboratory space

• Silent operation with 100% coverage of the MALDI analyte space

• Seamless switching between positive and negative ion modes

• Bruker smartbeam laser technology for enhanced sensitivity

• IR-laser-based automated source cleaning minimizes downtime

Typical applications:

• Routine MALDI-TOF analysis of small molecules to intact proteins

• Laboratories needing reliable, worry-free benchtop operation

• Facilities with space constraints requiring a compact MALDI system

The enhanced ultrafleXtreme: the industry standard MALDI-TOF/TOF mass spectrometer with 5 GS/s 10 bit digitizer for enhanced resolution and dynamic range

Fast and direct results for efficient research

Bruker's patented innovative smartbeam-II laser enables high data acquisition speed in both MS and MS/MS at full systems performance. The well-established proprietary smartbeam-II laser provides unprecedented analytical and matrix flexibility in workflows from protein tissue imaging, intact proteins analysis, glycoproteomics, biologics or oligo QC, polymer analysis, to LC-MALDI proteomics and TLC-MALDI – all fully enabled at 1-2000 Hz repetition rates.

Broadband mass resolving power up to 40,000 enables precision proteomics via Bruker’s unique PAN technology for highest mass resolution across a very broad mass range, not just at a selected optimum.

The FlashDetector combined with a 5 GS/s 10-bit digitizer and latest advances in electronics provide mass resolving power up to 40,000 and 1 ppm mass accuracy for highest confidence.

The IR-laser based self-cleaning ion source ensures robust, long-term highest-performance operation. Very long MALDI laser lifetime in combination with automated source cleaning in just minutes leads to high uptime and low maintenance costs.

Typically, full MS/MS data sets can be acquired with up to 1000 Hz laser repetition rate from low fmol levels within seconds. The Bruker bioinformatics software is perfectly adapted to analyze and visualize the match between the raw spectra and annotated peptide and protein structures.

The neofleX™ is a novel benchtop axial TOF/TOF designed for speed and flexibility within a small footprint for driving translational context for MALDI Imaging and beyond.

Easy-to-use: An ecosystem of consumables and softwareIlluminate: More insight per pixel for contextualizing tissue biologyRediscover: Enhanced speed and and performance in flexible MALDI workflowsTransform: Multiomic spatial biology applications from one slide

MALDI IMAGING FEATURES

Record breaking simultaneous imaging of 116 antibody probes

By leveraging MALDI Imaging add depth to the multiomic information from a single tissue section. Push your spatial biology further to understand the complex ecosystem of the tumor microenvironment. Ultimately, transform molecular pathway awareness into disease related insights with MALDI HiPLEX-IHC and other multiomic measurements from one tissue sample combined in a single software solution.

Our spatial biology toolkit empowers you to identify targets using disease specific HiPLEX experiments to assess target engagement effects. A groundbreaking 116-plex MALDI HiPLEX-IHC experiment with 30 µm spatial resolution was collected in 7 hours on squamous cell carcinoma. Comprehensive protein analysis enables differentiation of control tissue from small cell lung cancer types.