timsTOF
BRUKER timsTOF – Trapped Ion Mobility Time of Flight Mass Spectrometry:
Trapped Ion Mobility Spectrometry (TIMS) is an IMS technique where ions are propelled through the TIMS tunnel by a gas flow. An electrical field controls each ion from moving beyond a position defined by the ion’s mobility, where the push it experiences from the gas flow matches the force of the electrical field. Ramping down the electrical field allows selectively releasing ions from the TIMS tunnel according to their mobility.
By incorporating a TIMS device at the front of a quadrupole time-of-flight (QTOF) mass spectrometer, ions can be accumulated for a specific amount of time before being released for MS analysis. Using the PASEF® method, peptide ions are separated using TIMS, eluted (~ 100 ms) and detected in the QTOF, generating the TIMS MS heat map. In the PASEF® method, the same TIMS separation is used with the quadrupole isolating a certain ion species during its elution and immediately shifting to the next precursor. Parent and fragment spectra are aligned by mobility values.
TIMS
Trapped Ion Mobility Spectrometry (TIMS) is an IMS separation technique in gas phase, which resolves sample complexity with an added dimension of separation in addition to HPLC and mass spectrometry, increasing peak capacity and confidence in compound characterization. Equally as important, the TIMS device also serves to accumulate and concentrate ions of a given mass and mobility, enabling a unique increase in sensitivity and speed along with the additional dimension of separation.
A near 100% duty cycle for high sensitivity, high speed shotgun proteomics can now be achieved with the dual TIMS technology. The novel design allows for ions to be accumulated in the front section, while ions in the rear section are sequentially released depending on their ion mobility. This process is called Parallel Accumulation Serial Fragmentation, or PASEF®.
PASEF®
With speed in mind, Bruker experts redesigned MS/MS technology to meet the requirements of shotgun proteomics. With PASEF® technology >100 Hz sequencing speed can be achieved, and the MS/MS spectra quality of low abundant peptides can be increased by selecting them several times.
The timsTOF fleX is a fully functional high speed, high sensitivity ESI instrument for all your Omics analyses with integrated MALDI source for fast MALDI Imaging
High resolution imaging has never been easier. Bruker's microGRID technology combines MALDI stage and smartbeam 3D laser beam positioning to facilitate high quality imaging down to 5 µm spatial resolution.
The timsTOF Pro 2, powered by the latest parallel accumulation serial fragmentation (PASEF®) technology, paves the way for 4D-Multiomics applications. With its proven robustness, high sensitivity and fast MS/MS acquisition, timsTOF Pro 2 is the platform of choice for 4D-Proteomics™, 4D-Metabolomics™ and 4D-Lipidomics™.
Speed
PASEF® technology achieves a >120 Hz sequencing speed without affecting resolution.
Depth
Additional ion mobility dimension increases data completeness.
Robustness
The unique instrument design enables robust performance and routine operation over thousands of samples without requiring cleaning.
The timsTOF SCP for quantitative single cell biology research with unbiased, deep single-cell 4D-Proteomics™, immunopeptidomics, epiproteomics and PTM analysis to complement scRNA-seq. Expanding the horizons of single cell research.
Redefining single cell proteomics
Advanced ion optics and PASEF® to investigate cell heterogeneity and biology from a single cell
Mass spectrometric proteomics has become a staple of modern research in understanding biological function and disease mechanisms. Healthy or diseased tissues that seem homogenous are composed of cells with a variety of different proteomes. The challenge of deciphering the proteomes in each single cell – the cell heterogeneity – holds the key to fully understanding its function.
The timsTOF SCP offers a radically improved ion source concept. Combined with parallel accumulation serial fragmentation (PASEF®) acquisition methods, it provides extremely high speed and sensitivity to tackle proteomes of single cells or post translational modifications in a few cells that are morphologically or functionally similar.
Powered by the 4th generation TIMS-XR and advanced digitizer technology for high dynamic range and analytical depth in quantitative cell and tissue proteomics.
TIMS based ion mobility separation and CCS-enabled analysis
The new timsTOF HT mass spectrometer is equipped with the 4th generation high capacity TIMS-XR analyzer and advanced digitizer technology (ADT). It offers higher dynamic range for unmatched analytical depth and quantitation in high-throughput proteomics experiments. The timsTOF HT is fully CCS-enabled and supports all PASEF® acquisition modes including PASEF®, dia-PASEF® and prm-PASEF® achieving flexibility in 4D-Proteomics™. Simply uncompromised proteomic depth at scale.
Unleash 4D-Proteomics™ with the timsTOF Ultra 2 – the ultimate fusion of trapped ion mobility spectrometry (TIMS) with quadrupole time-of-flight (QTOF) technology.
Ultra sensitivity: TIMS PASEF® mode parallelization captures ions with near 100% efficiency, delivering exceptional sensitivity and unparalleled proteome depth at 300 Hz speeds.
Maximum ion transfer: Optimized CaptiveSpray Ultra 2 (CSI Ultra 2) ionization creates a vortex for optimal ion transfer and stable spray without adjustment.
Enhanced specificity: MOMA (Mobility Offset, Mass Aligned) allows identification and separation of co-eluting isobaric mass-to-charge ions in the mobility domain, increasing clarity and confidence.
Increased confidence: Confidence with low S/N peptide identification derived from CCS-Enabled TIMScore™, TIMS DIA-NN 4D-Proteomics™ or using Spectronaut® 19.
Upgrade your research platform for continuous innovation
We recognize the inherent duality of research investments: opportunity and risk. Embracing innovation's dynamism, we prioritize upgradable research platforms. This commitment fosters sustainability and empowers researchers to navigate the ever-evolving landscape.
Our upgradable platform seamlessly integrates the latest advancements, keeping your projects at the forefront of discovery. It grants researchers the flexibility to choose the optimal moment to leverage these advancements, maximizing the impact of their work and aligning perfectly with their research goals.
PASEF - Acquisistion
Revolutionize your research with PASEF® – for ultra-speed, ultra-sensitive 4D-Multiomics applications and robustness on timsTOF instruments. Discover new depths and insights.
Enhanced sensitivity: Combining advanced ion mobility technology, fragmentation processes, and sensitive detection capabilities to identify low-abundance peptides, metabolites, and lipids in complex biological samples.
Increased proteoform coverage: Identify and quantify a higher number of peptides to reveal more protein variants.
Faster data acquisition: PASEF® speeds up proteomics analysis by spreading sample complexity across the mobility dimension, TIMS ensures rich, high-quality data even at breakneck speeds. It's not just faster analysis, it's also better data.
Increased specificity: PASEF® empowers precision proteomics. MOMA boosts data clarity, letting you confidently untangle complex samples while maintaining blazing speed and sensitivity. No trade-offs, just superior insights.
See Beyond What’s Visible. Discover PASEF® Sensitivity and Speed
PASEF® (Parallel Accumulation Serial Fragmentation) is an innovative mass spectrometry technology that revolutionizes proteomics analysis. PASEF® is uniquely incorporated into Bruker's timsTOF mass spectrometers, which combine orthogonal ion mobility and time-of-flight separation to achieve unprecedented levels of selectivity, speed, and sensitivity.